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Fig. 6 (a and b) Western blot analysis of oxidative genes. (a) A2780 and (b) ID8 cells were incubated with ZnNP, ZnCl2, SNP (25 μg ml−1), and CisPt (3 µg ml−1; 10 µM) for 24 h. (c–f) Cytotoxicity of ZnNP, ZnCl2, SNP and CisPt on healthy liver and PDTOs obtained from HGSOC patients (Pat A–C). Error bars represent the standard error of the mean (SEM). Groups were considered statistically significant if p < 0.05 (*), p < 0.01 (**) or non-signifi- cant (ns). (g) Hematoxylin & eosin staining and immunohistochemistry (PAT <t>A–C).</t> <t>CA-125</t> (cancer antigen 125), WT1 (Wilms’ tumor 1) and PAX8 (paired box gene 8) are markers of ovarian cancer (all images 40×, bar = 50 µm).
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Fig. 6 (a and b) Western blot analysis of oxidative genes. (a) A2780 and (b) ID8 cells were incubated with ZnNP, ZnCl2, SNP (25 μg ml−1), and CisPt (3 µg ml−1; 10 µM) for 24 h. (c–f) Cytotoxicity of ZnNP, ZnCl2, SNP and CisPt on healthy liver and PDTOs obtained from HGSOC patients (Pat A–C). Error bars represent the standard error of the mean (SEM). Groups were considered statistically significant if p < 0.05 (*), p < 0.01 (**) or non-signifi- cant (ns). (g) Hematoxylin & eosin staining and immunohistochemistry (PAT <t>A–C).</t> <t>CA-125</t> (cancer antigen 125), WT1 (Wilms’ tumor 1) and PAX8 (paired box gene 8) are markers of ovarian cancer (all images 40×, bar = 50 µm).
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Fig. 6 (a and b) Western blot analysis of oxidative genes. (a) A2780 and (b) ID8 cells were incubated with ZnNP, ZnCl2, SNP (25 μg ml−1), and CisPt (3 µg ml−1; 10 µM) for 24 h. (c–f) Cytotoxicity of ZnNP, ZnCl2, SNP and CisPt on healthy liver and PDTOs obtained from HGSOC patients (Pat A–C). Error bars represent the standard error of the mean (SEM). Groups were considered statistically significant if p < 0.05 (*), p < 0.01 (**) or non-signifi- cant (ns). (g) Hematoxylin & eosin staining and immunohistochemistry (PAT <t>A–C).</t> <t>CA-125</t> (cancer antigen 125), WT1 (Wilms’ tumor 1) and PAX8 (paired box gene 8) are markers of ovarian cancer (all images 40×, bar = 50 µm).
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Fig. 6 (a and b) Western blot analysis of oxidative genes. (a) A2780 and (b) ID8 cells were incubated with ZnNP, ZnCl2, SNP (25 μg ml−1), and CisPt (3 µg ml−1; 10 µM) for 24 h. (c–f) Cytotoxicity of ZnNP, ZnCl2, SNP and CisPt on healthy liver and PDTOs obtained from HGSOC patients (Pat A–C). Error bars represent the standard error of the mean (SEM). Groups were considered statistically significant if p < 0.05 (*), p < 0.01 (**) or non-signifi- cant (ns). (g) Hematoxylin & eosin staining and immunohistochemistry (PAT <t>A–C).</t> <t>CA-125</t> (cancer antigen 125), WT1 (Wilms’ tumor 1) and PAX8 (paired box gene 8) are markers of ovarian cancer (all images 40×, bar = 50 µm).
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Fig. 6 (a and b) Western blot analysis of oxidative genes. (a) A2780 and (b) ID8 cells were incubated with ZnNP, ZnCl2, SNP (25 μg ml−1), and CisPt (3 µg ml−1; 10 µM) for 24 h. (c–f) Cytotoxicity of ZnNP, ZnCl2, SNP and CisPt on healthy liver and PDTOs obtained from HGSOC patients (Pat A–C). Error bars represent the standard error of the mean (SEM). Groups were considered statistically significant if p < 0.05 (*), p < 0.01 (**) or non-signifi- cant (ns). (g) Hematoxylin & eosin staining and immunohistochemistry (PAT <t>A–C).</t> <t>CA-125</t> (cancer antigen 125), WT1 (Wilms’ tumor 1) and PAX8 (paired box gene 8) are markers of ovarian cancer (all images 40×, bar = 50 µm).
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Image Search Results


Journal: Cell reports

Article Title: PAI-1 uncouples integrin-β1 from restrain by membrane-bound β-catenin to promote collagen fibril remodeling in obesity-related neoplasms

doi: 10.1016/j.celrep.2024.114527

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-CA125 , Biocare Medical , Cat# CM 101 AK, CK.

Techniques: Recombinant, Membrane, Cell Culture, Reverse Transcription, SYBR Green Assay, Clinical Proteomics, Protein Extraction, Extraction, Bicinchoninic Acid Protein Assay, In Situ, Blocking Assay, Migration, shRNA, Control, Software, Pyromark Assay, Western Blot, Simple Western

Journal: Cell reports

Article Title: PAI-1 uncouples integrin-β1 from restrain by membrane-bound β-catenin to promote collagen fibril remodeling in obesity-related neoplasms

doi: 10.1016/j.celrep.2024.114527

Figure Lengend Snippet:

Article Snippet: The sections were incubated with rabbit polyclonal anti-estrogen receptor alpha (1: 300, Santa Cruz Biotechnology, Cat# sc-542), rabbit polyclonal anti-progesterone receptor (1:100, Dako, Cat# A0098), mouse monoclonal anti-CA125 (1:100, Biocare Medical, Cat# CM 101 AK, CK), mouse monoclonal anti-p53 (IHC World, Cat# IW-MA1078), rabbit polyclonal anti-WT1 (1:2000, Santa Cruz Biotechnology, Cat# sc-192), goat polyclonal anti-β-catenin (1:200, Santa Cruz Biotechnology, Cat# sc-1496), mouse monoclonal anti-E-cadherin (1:100, Zymed Laboratories Inc, Cat# 18–0223) or mouse monoclonal anti-Ki67 (1:100, Dako, Cat# M7240) for 1 h at room temperature.

Techniques: Recombinant, Membrane, Cell Culture, Reverse Transcription, SYBR Green Assay, Clinical Proteomics, Protein Extraction, Extraction, Bicinchoninic Acid Protein Assay, In Situ, Blocking Assay, Migration, shRNA, Control, Software, Pyromark Assay, Western Blot, Simple Western

Fig. 6 (a and b) Western blot analysis of oxidative genes. (a) A2780 and (b) ID8 cells were incubated with ZnNP, ZnCl2, SNP (25 μg ml−1), and CisPt (3 µg ml−1; 10 µM) for 24 h. (c–f) Cytotoxicity of ZnNP, ZnCl2, SNP and CisPt on healthy liver and PDTOs obtained from HGSOC patients (Pat A–C). Error bars represent the standard error of the mean (SEM). Groups were considered statistically significant if p < 0.05 (*), p < 0.01 (**) or non-signifi- cant (ns). (g) Hematoxylin & eosin staining and immunohistochemistry (PAT A–C). CA-125 (cancer antigen 125), WT1 (Wilms’ tumor 1) and PAX8 (paired box gene 8) are markers of ovarian cancer (all images 40×, bar = 50 µm).

Journal: Biomaterials science

Article Title: Self-targeted nanosystem for enhanced chemodynamic cancer therapy.

doi: 10.1039/d4bm01683a

Figure Lengend Snippet: Fig. 6 (a and b) Western blot analysis of oxidative genes. (a) A2780 and (b) ID8 cells were incubated with ZnNP, ZnCl2, SNP (25 μg ml−1), and CisPt (3 µg ml−1; 10 µM) for 24 h. (c–f) Cytotoxicity of ZnNP, ZnCl2, SNP and CisPt on healthy liver and PDTOs obtained from HGSOC patients (Pat A–C). Error bars represent the standard error of the mean (SEM). Groups were considered statistically significant if p < 0.05 (*), p < 0.01 (**) or non-signifi- cant (ns). (g) Hematoxylin & eosin staining and immunohistochemistry (PAT A–C). CA-125 (cancer antigen 125), WT1 (Wilms’ tumor 1) and PAX8 (paired box gene 8) are markers of ovarian cancer (all images 40×, bar = 50 µm).

Article Snippet: The following antibodies were used to characterize PDTO and parental tumours: PAX8 (catalog no.: 10336-1-AP, dilution 1 : 400, ProteinTech Group, Planegg-Martinsried, Germany), CA125 (catalog no.: sc-52095, dilution 1 : 100, Santa Cruz Biotechnology, Dallas, TX, USA) and WT1 (catalog no.: ab89901, dilution 1 : 300, Abcam, Cambridge, UK).

Techniques: Western Blot, Incubation, IF-P, Staining, Immunohistochemistry, Wilms Tumor Assay